Hoefer SE900 - Manual (Page 14)

• p8
Multiple Gel Caster
The multiple gel caster is included with the SE900-1.0 (Fig 10), and
can be ordered separately as the SE915.
The multiple gel caster is used to cast up to seven 1 mm thick gels
at the same time. Separator sheets are placed between glass cassettes
before casting to keep the cassettes from sticking to each other after
polymerization.
When casting single percentage gels by hand, pour the gel solution
down the channel in the back wall of the caster (Fig 11). The cassettes
will fill from the bottom producing better quality gels, and reducing
the chances of trapping air bubbles.
When casting gradient gels, the gel solution should be slowly pumped
in through the port at the bottom. The triangular plug at the bottom
should be removed. The triangular region forms a space for the gradi-
ent gel to spread out and enter the glass cassettes uniformly.
When using butanol overlays, try to minimize contact of butanol with
the plastic multicaster. Prolonged contact with butanol may craze the
plastic of the multicaster.
Space Saver
The space saver is used to fill space within the multiple gel caster, and
reduce the amount of gel solution. One space saver is equivalent in
thickness to one 1.0 mm glass cassette.
When casting six 1.0 mm thick gels, use one space saver at the back
of the multiple gel caster.
One space saver is included with the multiple gel caster. Additional
space savers (SE912) can be ordered separately if less than 6 gels are
routinely cast.
Fig 10. Multiple gel caster.
Fig 11. Multicaster filling line.