Hoefer SE900 User Manual

Page 23

• p17

Fig 20. Gel orientation.

Fig 21. Slide safety lid into place.

Note: The black high voltage lead should be
on the same side as the IPG strip.

Black High Voltage Lead
Black Safety Interlock

–

Proper Fill Level

IPG Strip

Rubber Hinge

Red High Voltage Lead
Red Safety Interlock

After all gels are placed in the tank, check the level of buffer in the
separation tank.

The proper level is about halfway up the spacer on the

top edge of the gel cassettte. The buffer level also must be below the
maximum fill line.

Attach the safety lid to the separation tank in the proper orientation
by moving and fitting the top of the safety interlocks through the slots
on the lid, and connecting the high voltage leads to the banana plugs.
The safety interlock will secure the lid in place (Fig 21).

Connect the high voltage leads to a power supply capable of delivering
at least 300 V, 500 mA, 90 W, such as the Hoefer PS300-B. Adapters
may be needed if using other power supplies.

10 Set the power supply to the desired values and start the run.

Note: For day runs set the power supply for 80 mA/gel. When running
a full tank of 6 gels set the power supply for 480 mA. Runs should
be complete in about 6 hours. The voltage and wattage should be set
to their maximums, 600 V and 100 W respectively, so as not to limit
the current.

For overnight runs (18 hours), set the power supply for 80 V constant.
The current and wattage should be set not to exceed 500 mA and
100 W.

11 The run is complete when the tracking dye has reached the opposite

side of the gels.

12 Turn off the power supply, the circulation pumps, and the external

recirculating water bath, if being used.