Gel recipes – Hoefer SE900 User Manual
Page 34
• p28
Gel Recipes
The Laemmli gel recipes are for 400 ml of a single concentration solu-
tion (enough for six 1.0 mm, 25 × 20 cm gels). Tabulated are volumes
for relatively large pore gels (10% T range) as well as smaller pore
gels (12.5–20% T range). 5% and 7.5% gels are difficult to handle in
large format gels but can be blended into gradient gels for better reso-
lution and easier handling. The recipes given here are for guidance
when casting gradient gels. Use ½ total required volume each solution
when casting gradient gels. Using the solutions given in Appendix A,
all gels are crosslinked with 2.6% C.
Laemmli Gel (per 400 ml gel solution)
Resolving Gel Solution
400 ml
10.0%
12.5% 15.0% 17.5% 20%
Acrylamide Stock (Soln. #1)
133.3 166.7 200.0 233.3 266.7
1.5 M TrisCl, pH 8.8 (Soln. #2) 100.0 100.0 100.0 100.0 100.0
10% SDS (Soln. #3)
4.0
4.0
4.0
4.0
4.0
Deionized H
2
O
158.0 124.8
91.5
58.3
25.0
10% APS (Soln. #4)
4.0
4.0
4.0
4.0
4.0
10% TEMED (Soln. #5)
0.68
0.55
0.45
0.40
0.34
Final Volume
400.0 400.0 400.0 400.0 400.0