Hoefer SE900 - Manual (Page 35)

• p29
Laemmli Gel (per 200 ml each gel solution)
Gradient Gels Solution
200 ml LIGHT
5.0% 7.5% 10.0% 12.5% 15.0% 17.5%
Acrylamide Stock (Soln. #1) 33.3 50.0 66.7 83.3 100.0 116.7
1.5 M TrisCl, 50.0 50.0 50.0 50.0 50.0 50.0
pH 8.8 (Soln. #2)
10% SDS (Soln. #3) 2.0 2.0 2.0 2.0 2.0 2.0
Deionized H
2
O 112.2 95.5 79.0 62.4 45.8 29.1
10% APS (Soln. #4) 2.0 2.0 2.0 2.0 2.0 2.0
10% TEMED (Soln. #5) 0.50 0.46 0.34 0.27 0.23 0.20
Final Volume 200.0 200.0 200.0 200.0 200.0 200.0
200 ml HEAVY
7.5% 10.0% 12.5% 15.0% 17.5% 20%
Acrylamide Stock (Soln. #1) 50.0 66.7 83.3 100.0 116.7 133.3
1.5 M TrisCl, 50.0 50.0 50.0 50.0 50.0 50.0
pH 8.8 (Soln. #2)
10% SDS (Soln. #3) 2.0 2.0 2.0 2.0 2.0 2.0
Deionized H
2
O 83.0 66.5 49.9 33.3 16.6 0.0
10% APS (Soln. #4) 2.0 2.0 2.0 2.0 2.0 2.0
10% TEMED (Soln. #5) 0.46 0.34 0.27 0.23 0.20 0.17
Glycerol 12.50 12.50 12.50 12.50 12.50 12.50
Final Volume 200.0 200.0 200.0 200.0 200.0 200.0