Hoefer SE900 - Manual (Page 24)

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After Electrophoresis
1 Unplug the leads to the power supply.
2 Remove the lid by moving the safety interlocks while lifting the lid.
3 Carefully lift out each gel. The cassettes are slippery.
Do not use the PAGE rack as a gel carrying rack as the plates may
slide out of the rubber gaskets when tipped or moved.
4 Place the glass cassette on a clean, flat surface.
5 Use the Wonder Wedge to gently open the nonhinged side of the
gel cassette.
6 Use the Wonder Wedge to gently cut the gel away from the side
spacers.
7 Carefully remove the gel from the cassette and place in desired
staining solutions.
8 Attach a length of tubing long enough to reach a sink to the drain
connector (Fig 22). Insert the connector into the drain port on the
lower left side of the SE900, behind the black safety interlock.
9 Allow the tank to drain by gravity. The tank will drain best by having
the drain line at least 18 inches (50 cm) below the tank.
10 Once drained, re-insert empty PAGE rack. Fill the tank with deionied
water, turn on pumps and circulate for 5–10 minutes.
11 Turn off pumps and drain again.
12 Remove the PAGE rack and allow the components to air dry.
13 Remove and rinse any residual acrylamide from the drain filter at the
bottom of the separation tank. Replace the drain filter so that it is
below flush.
Note: DO NOT use the buffer circulation
pumps to help the tank drain faster.
Fig 22. Drain port.