Hoefer SE900 - Manual (Page 21)

• p15
Sealing the IPG to the Second Dimension Gel
1 Remove residual liquid from the top of the second dimension gel by
tipping the cassette and blotting one corner with a lint-free tissue.
2 Apply the equilibrated IPG strip to the gel surface (Fig 17). A thin
plastic strip is useful to gently seat the IPG directly against the top of
the second dimension gel. Do not press the strip into the gel or the
results may be distorted. Avoid trapping air bubbles between the strip
and the second dimension gel.
3 Seal the IPG strip in place by applying hot, molten agarose over the
IPG strip (Fig 18). See Appendix A for suggested recipe. Avoid intro-
ducing air bubbles while applying the overlay.
4 Allow the agarose to solidify before placing gels into the
separation tank.
Fig 17. Applying IPG strip to second
dimension gel.
Fig 18. Adding agarose overlay.