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Hanna Instruments HI 83414 User Manual

Page 19

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19

ADDING REAGENT (COLORIMETRY only)
• Because the reagent quantity is set up to react with 10 mL of

sample, it is very important to fill the cuvette correctly. The
liquid in the cuvette forms a meniscus; the bottom of the meniscus
must be at the same level with as the 10 mL mark.

• To open the powder reagent pack:

a) use scissors to open the powder packet
b) push the edges of the packet to form a spout
c) pour the contents of the packet

• Do not let the reacted sample stand too long after reagent is added or accuracy will be lost.
• All the reaction times reported in this manual were recorded at 20 ºC (68 ºF). As a general rule

of thumb, they should be doubled at 10 ºC (50 ºF) and halved at 30 ºC (86 ºF).

• Insert the cuvette with the mark aligned with the mark on the instrument top.
• It is possible to take multiple readings in a row but it is recommended to take a new zero reading

for each sample and to use the same cuvette for zeroing and measurement.

• After the reading, it is important to discard the sample immediately, otherwise the glass might

become permanently stained.

Note: In order to maximize accuracy prior to a measurement, follow the validation procedure to

be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.