Shellab SRI20P - Manual (Page 19)

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OPERATION (CONTINUED)
C ALIBRATE THE T EMPERATURE DISPLAY
Allow the incubator at least 24 hours to stabilize at its operational set point before performing a
calibration. Always use a certified temperature sensing reference device that is regularly calibrated to
0.1°C by an independent party to conduct temperature verifications or calibrations. For best results use a
remote sensing device.
1. Place the temperature sensor of a calibrated reference device inside the incubator as close as
possible to the chamber’s geometric center. Check that the sensor is not in direct contact with the
shelving.
Note: A thermocouple sensor probe’s sleeve may be taped to the shelving, as long as the exposed copper
end is 2 inches (5cm) above the shelf. An exposed sensor probe in direct contact with the shelving
may experience heat sinking, which can result in an inaccurate temperature reading.
2. Allow the temperature to re-stabilize after closing the door. The reference device ’s temperature
measurement should not change for at least one (1) hour after a significant disruption for the
chamber to be considered stabilized.
3. If the reference device’s and the incubator’s temperature readings are the same, or the difference
between the two falls within the acceptable range of your laboratory protocol after one (1) hour of
stability, the incubator is calibrated.
4. If there is a difference between the two (2) readings, and that offset falls outside your laboratory
protocol ’s acceptable range, adjust the incubator’s temperature display to match the reference
device’s reading. See the next step. If the door was briefly opened to take a reading, wait fifteen
minutes for the temperature to stabilize before correcting for the offset.
5. Press and hold both the UP and DOWN arrows simultaneously. The Temperature Display will
begin flashing and display the current temperature value. Adjust the current value until it
matches the reference device’s temperature reading. This will enter the offset into the unit’s
temperature controller board.
6. After matching the unit’s current value to that of the reference device, wait five seconds. The
incubator’s Temperature Display will cease flashing, and the incubator will begin heating or
cooling to compensate for the entered temperature offset.
7. Allow the incubator one (1) hour to stabilize after reaching the new temperature.
8. Compare the reference device’s reading with the incubator’s. If the reference device’s and the
incubator’s temperature readings are the same or fall within the range of your laboratory protocol,
the incubator is now calibrated.
9. If the two readings are not the same or fall outside your laboratory protocol, repeat steps 5 – 8.
10. If the temperature readings of the incubator and the reference device still fall outside your
laboratory protocol after three calibration attempts, contact Sheldon Technical Support for
assistance.