Shellab SCO31 - Manual (Page 24)

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OPERATION (CONTINUED)
C ALIBRATE THE T EMPERATURE DISPLAY
This procedure requires a temperature reference sensor device. Always use a reference device
calibrated to at least 0.1°C. For best results use a digital device with a wire sensor probe.
Temperature calibrations are performed to ensure that the incubator temperature display matches
the actual air temperature inside the incubation chamber. Calibrate as often as required by your
laboratory protocol or regulatory compliance. Sheldon Manufacturing strongly recommends
performing a temperature calibration when installing the incubator in a new environment.
Allow the incubator to stand for at least 8 hours undisturbed prior to this procedure, running at its
operational set point in order to stabilize with no temperature fluctuations greater than ± 0.1°C.
Failure to allow at least 8 hours will result in an inaccurate temperature calibration.
Procedure continued on next page
1. If you have not already done so, place the temperature sensor of the
reference device inside the incubator as close as possible to the
chamber’s geometric center. Check that the sensor is not in direct contact
with the shelving.
Note:
A thermocouple sensor probe’s sleeve may be taped to the shelving, as long
as the exposed copper end is 2 inches (5cm) above the shelf. An exposed sensor
probe in direct contact with the shelving may experience heat sinking, which can
result in an inaccurate temperature reading.
Note: Sensor probes may
be introduced into the
chamber through the door,
or on the SCO58 through
the access port. Place the
port stopper back into the
port after introducing the
probe.
2. Allow the temperature to re-stabilize if the door has been opened.
a. The reference device temperature measurement should not
change for at least one (1) hour in order for the chamber to be
considered re-stabilized.
3. Once the temperature has stabilized, compare the reference device and
the incubator display temperature readings. If the readings are the same,
or the difference between the two falls within the acceptable range of your
laboratory protocol, the incubator is calibrated for temperature. The
Temperature Calibration procedure is complete.
Reference Device
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4. If there is a difference between the two (2) readings, and that offset falls
outside your laboratory protocol ’s acceptable range, place the SCO6AD
into calibration mode. See the next step.
a. Before placing the unit in calibration mode, allow the
temperature to re-stabilize if the door has been opened. This
will require a wait of at least one (1) hour. Failure to do so will
result in an inaccurate calibration.
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