BUCHI NIRWare User Manual

Page 29

NIRWare 1.5 Software Manual

Tutorial

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The application should be able to check the identity of four different types / forms of sugar: Fructose (=
fruit sugar), lactose (= milk sugar), sucrose (= crystal sugar) and fine sucrose (= sugar powder).

Note that sucrose and fine sucrose are of the same chemical composition and only differ in particle size.

The process of creating a ready-to-use NIR application requires reference samples. These are samples of
doubtless identity and quality. In combination with their N

IR spectra (‘reference spectra’) they provide the

necessary data sets for calculating a chemometric calibration. The calibration can then be used for
prediction, which means deriving the identity information from the spectra of substances to be tested.

A typical use of an NIR application with such a calibration is the identity check of incoming substances in
a pharmaceutical plant.

It is recommended to use dedicated applications for reference measurement and routine use (=
prediction).

NOTE

The terms 'reference measurement', 'reference samples' or 'reference spectra' used in the tutorial
describes the data collected for calibration development.

To ensure that reference and unknown samples are measured under identical spectrometric conditions,
we suggest to start the development of the routine application with a copy of the application for reference
measurement, which is then adjusted.

Please keep in mind that only applications in the ‘Approved’ Lifecycle state are visible to (and usable by) a
user fro

m the user group ‘Operators’.

These considerations are visualized in the following scheme: