Bio-Rad Nuvia™ HR-S Media - Manual (Page 9)

Nuvia HR-S Instruction Manual 5
Packing Process-Scale Columns
After removing the storage buffer (Section 3), prepare
a 20–50% slurry (v/v) with packing buffer (see Table 2).
Follow the column manufacturer’s recommendations with
one major exception: do not recirculate the Nuvia slurry
through the packing pump.
Use a low-shear hydrofoil impeller for automated mixing
or a plastic paddle for manual mixing. The best overall
performance of Nuvia HR-S will be obtained with a
compression factor of 1.25, defined as settled bed height
divided by the packed bed height.
Bio-Rad recommends using process columns with axial
compression capabilities for best results. For stall-packing
type columns, additional compression may be required to
achieve a compression factor of 1.25.
After the desired compression is achieved, flow condition
the column with fresh packing or equilibration buffer for
3 column volumes (CV) in upflow followed by 3 CV in
downflow at the process flow rate. After flow conditioning,
evaluate column efficiency using your standard operating
procedures or the procedure described in Section 5.
Detailed packing procedures for process scale
columns can be obtained by contacting your Bio-Rad
representative.