Bio-Rad Nuvia™ HR-S Media - Manual (Page 12)

8 Nuvia HR-S Instruction Manual
All buffers commonly used for ion exchange
chromatography can be used with Nuvia media
(see Table 2). Buffering ions that have the same charge as the
functional group on the ion exchanger will produce the best
results.
Table 2. Common buffers for ion exchange
chromatography.
Buffer Buffering Range
Acetic acid 4.8–5.2
Citric acid 4.2–5.2
HEPES 6.8–8.2
Lactic acid 3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
TES 6.8–8.2
Tricine 7.8–8.9