Bio-Rad Nuvia™ HR-S Media - Manual (Page 13)

Nuvia HR-S Instruction Manual 9
Section 7 : Regeneration and
Sanitization
After each run, the packed bed should be washed with 2–6
bed volumes of 1–2 M NaCl or until absorbance returns to
baseline to remove reversibly bound material. The column can
then be sanitized in 1.0 N NaOH at 50–100 cm/hr; a minimum
contact time of 120 min is recommended.
Section 8 : Cleaning in Place
(CIP) and Sanitization
If a column no longer yields reproducible results, the
media may require thorough CIP and sanitization after
regeneration to remove strongly bound contaminants.
Acceptable CIP agents include 25% acetic acid, 8 M urea,
1% Triton X-100, 6 M potassium thiocyanate, 70%
ethanol, 30% isopropyl alcohol, 1 N NaOH, 6 M guanidine
hydrochloride, and 1 to 5% SDS.
Section 9 : Storage
For long-term storage, Nuvia HR-S media should be
equilibrated with 0.01–0.1 N NaOH or 20% ethanol
(see Table 1).
Section 10 : Regulatory Support
Regulatory support files are available for Nuvia HR-S
media. If you need assistance validating the use of Nuvia
media in a production process, contact your local Bio-Rad
representative.