Bio-Rad Nuvia™ HR-S Media - Manual (Page 8)

4 Nuvia HR-S Instruction Manual
Section 4 : Column Packing
Nuvia HR-S media can be packed using most
conventional packing methods. A slurry concentration of
20–50% is recommended. The compression factor for
Nuvia HR-S is 1.25. The compression factor is defined as
settled bed height divided by packed bed height.
Packing Laboratory Scale Columns
This slurry packing method was designed to pack
Nuvia HR-S media in a conventional column with an
internal diameter of 5–15 mm. All buffers should be
degassed. Because a relatively large volume of slurry is
required, it is recommended that a packing reservoir be
used.
1. Prepare degassed 1.0 M NaCl, 20–50 mM buffer salt
(see Table 2), referred to hereunder as the packing
buffer.
2. Nuvia media are shipped as a 50% slurry. Measure the
desired amount of suspended slurry into a graduated
cylinder. Allow the resin bed to settle. Decant the
shipping solution from the settled media.
3. Add sufficient degassed packing buffer to the resin to
make a 20–50% slurry.
4. Mix to suspend the resin. Caution: Do not mix with
a magnetic stir bar as damage may occur. Larger
amounts of slurry may be mixed with a low-shear
marine impeller at low to moderate speed.
5. Add packing buffer to the column to about 2–5 cm
high. Pour in prepared resin slurry.
6. Once a layer of supernatant has developed, insert the
column flow adaptor and flow pack at a linear velocity
of 300–600 cm/hr with packing buffer for at least
10 min. Lower the adaptor to the calculated
compressed bed height.