Bio-Rad Nuvia™ HR-S Media User Manual
Page 7
Nuvia HR-S Instruction Manual 3
Section 3
:
Preparation
Nuvia
HR-S media is supplied fully hydrated in 20%
ethanol + 1 M NaCI as a 50% (v/v) slurry. For column
packing, removal of the shipping buffer is recommended.
Small volumes of Nuvia HR-S media are easily washed in
a Büchner funnel with 4–5 volumes of packing buffer. For
large volumes, cycling through 3–4 settling and decanting
steps using the packing buffer is recommended.
Removal of fines from Nuvia HR-S media is not required
due to the narrow particle-size range. If fines have been
generated during handling, resuspend the slurry and
remove the opaque supernatant before sedimentation is
complete. Repeat several times until supernatant is clear.
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