Bio-Rad Nuvia™ HR-S Media - Manual (Page 11)

Nuvia HR-S Instruction Manual 7
Section 6 : Operation and
Maintenance
A linear flow rate of 100 cm/hr with a 20 cm bed is a
recommended starting point for Nuvia HR-S. Purification
may be optimized by changing the pH, flow rate, or ionic
strength of the elution buffer, modifying the gradient profile,
or experimenting with different buffer salts.
Note: Due to the smaller particle size of Nuvia HR-S
media, it is recommended to run the process at flow rates
no greater than 200 cm/hr to minimize backpressure and
headspace formation.
Figure 1 shows the effect of flow rate on backpressure.
Fig. 1. Nuvia HR-S media pressure/flow performance for a 20 cm
diameter x 20 cm bed height column packed to a compression
factor of 1.25.