Bio-Rad Nuvia™ HR-S Media - Manual (Page 6)

2 Nuvia HR-S Instruction Manual
Section 2 : Technical Description
Table 1. Characteristics of Nuvia HR-S media.
Nuvia HR-S
Type of ion exchanger Strong cation
Functional group –SO3 –
Total ionic capacity 100–180 µeq/ml
Dynamic binding capacity* >70 mg/ml at 300 cm/hr
Shipping counterion Na+
Median particle size 50 ± 10 µm
Recommended linear flow
rate range
50–200 cm/hr
Chemical stability —
1.0 N NaOH (20°C) up to 5 weeks
0.1 N NaOH (20°C)** up to 5 years
Compression factor 1.25 (settled bed
volume/packed bed volume)
pH stability** 2–14 short term
4–13 long term
Shipping solution 20% ethanol
Regeneration 1–2 M NaCl
Sanitization 0.5–1.0 N NaOH
Storage conditions 20% ethanol or 0.1 N NaOH
* 10% breakthrough capacity determined with 5.0 mg/ml human IgG in 20 mM
NaOAc pH 5.0
** Data derived under accelerated conditions at 60°C.