Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 6)

Section 3
Storage Conditions
All kit components (including lyophilized DNase I) should be stored at room
temperature. Store reconstituted DNase I at –20°C in a nonfrost-free freezer,
avoiding repeated freeze-thaw cycles. If precipitation is observed in any
solution, warm the solution to 37°C to redissolve and allow the solution to
return to room temperature before use.
Section 4
Necessary Supplies
Equipment and reagents to be provided by the customer:
• Microcentrifuge (>12,000 x g)
•
b-mercaptoethanol, 14.2 M (catalog # 161-0710)
• Lyticase (for yeast RNA isolation only)
• Lysozyme (for bacterial RNA isolation only)
• Isopropanol (for bacterial RNA isolation only)
• Polyvinylpyrrolidone-40 (PVP), 2% (for plant isolation only)
• 95–100% ethanol
• Tris for DNase I reconstitution (catalog # 161-0716)
Additional equipment required for vacuum format:
• Aurum™ vacuum manifold with vacuum regulator and column adaptor plate
(catalog # 732-6470), or other vacuum manifold with luer fittings
• Vacuum source (capability of –23 inHg required)
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