Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 11)

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Table 2. Disruption and homogenization methods.
Starting Disruption Homogenization
Material Method Method
Cultured mammalian Lysis solution Pipetting up and down
cells 18-gauge needle and syringe
Bacteria Lysozyme Pipetting up and down
+ lysis solution 18-gauge needle and syringe
Yeast Lyticase Pipetting up and down
+ lysis solution 18-gauge needle and syringe
Animal tissue Mortar and pestle Rotor-stator homogenizer*
+ lysis solution Pipetting up and down
18-gauge needle and syringe
Plant tissue Mortar and pestle Rotor-stator homogenizer*
+ lysis solution Pipetting up and down
18-gauge needle and syringe
*Rotor-stator homogenizers are recommended for animal and plant tissue.
• Mortar and pestle: freeze the tissue with liquid nitrogen, then grind it into a
fine powder under liquid nitrogen
• Pipetting up and down: pass the lysate through a standard micropipettor tip
several times
• 18-gauge needle and syringe: pass the lysate through the needle several
times
• Rotor-stator homogenizer: immerse the tip of the homogenizer into the
solution and homogenize for 30–60 sec
If column clogging occurs, switching to a more vigorous homogenization
method may lower the incidence of column clogging
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