Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 14)

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Manifold Wash Setup (Figure 2)
1. Insert the CAP (luer ends up) into the depression in the vacuum manifold
top. Ensure that the CAP rests evenly on the gasket.
2. Insert the luer ends of the desired columns into the available luer fittings,
ensuring a tight fit.
3. Close the unused luer fittings with the caps provided. Close caps by
rotating clockwise until light resistance is encountered. Excessive
tightening of a cap may cause the luer fitting to dislodge when the cap is
removed.
4. The manifold is now ready for column processing according to the
vacuum protocol of the appropriate column purification kit.
5. When ready to elute, proceed with the appropriate spin elution step as
recommended by the protocol.
6. After finishing the elution, rinse the CAP and Aurum vacuum manifold
with water and air dry or wipe with paper towels.
Fig. 2. Manifold setup for column processing.
CAP
Manifold top
Manifold base
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