Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 12)

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Section 6
Vacuum Manifold Setup and Use With
the Column Adaptor Plate (CAP)
Guidelines for Vacuum Format
• The recommended operating range is –17 to –23 inHg. Do not exceed
–25 inHg when performing this protocol. A vacuum regulator is strongly
recommended to establish the appropriate negative pressure
Table 3. Pressure unit conversions.
To convert from inches of mercury (inHg) to: Multiply by:
millimeters of mercury or torr (mmHg, torr) 25.4
millibar (mbar) 33.85
atmospheres (atm) 0.03342
pounds per square inch (psi) 0.4912
kilopascals (kPa) 3.385
About The CAP
The Aurum™ column adaptor plate (CAP) interfaces with the Aurum vacuum
manifold to convert the manifold from a plate- to a column-processing system.
The CAP has 18 luer fittings in a 6 x 3 array and comes supplied with luer
caps. Up to 18 Aurum miniprep columns can be accommodated on the CAP
without the need for connectors or other manifold accessories. The CAP will
also accommodate other columns with luer ends.
When vacuum is applied to the manifold, the CAP should self-seat, forming an
airtight seal without the need to press down it. However, the application of
gentle downward force may occasionally be required to facilitate seating.
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