Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 25)

Problem Possible Cause Recommended Solution
Total RNA prep Incorrect use of wash Add the appropriate
performs poorly solutions volume of 95–100%
in downstream ethanol to the wash
applications solutions before initial
use
RNA is degraded See problem “RNA
degradation”
Ethanol contamination Add 1–3 min to the
in prep (eluate volumes centrifugation time
>80 µl) after the final wash step
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