Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 5)

Section 1
Introduction
The Aurum™ total RNA mini kit purifies total RNA samples rapidly from
mammalian cell cultures, bacteria, and yeast (Saccharomyces cerevisiae), as
well as animal and plant tissue. Total RNA samples prepared using the Aurum
total RNA mini kit are suitable for use in a variety of downstream applications,
including reverse transcription-PCR (RT-PCR), real-time PCR, and northern
blots. A DNase l digest during the purification effectively removes genomic
DNA contamination from the preparation, eliminating the need for separate
DNase digests. All solutions and binding columns in the kit are RNase-free,
ensuring the integrity of the isolated total RNA. The Aurum total RNA mini kit
may be used in a spin or vacuum format using the Aurum vacuum manifold
(catalog # 732-6470).
Section 2
Kit Components
The Aurum™ total RNA mini kit contains the following components:
Components* Quantity/Amount
RNA binding columns 50
Capless wash tubes, 2 ml 50
Capped microcentrifuge tubes, 1.5 ml 50
Capped microcentrifuge tubes, 2.0 ml 100
DNase I (lyophilized) 1 vial
Lysis solution 50 ml
Low stringency wash solution
(5x concentrate)
20 ml
High stringency wash solution 40 ml
Elution solution 20 ml
DNase dilution solution 20 ml
*There may be reagent remaining in some bottles.
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