Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 13)

9
Preparing the Aurum Vacuum Manifold
Tubing provided in the Aurum vacuum manifold kit (catalog # 732-6470) is 4 ft
long and must be cut into appropriate pieces before proceeding.
Prior to setup, you may ensure that the gauge pointer is adjusted to zero by
removing the lens cover, followed by turning the adjustment pin located beneath
the dial face.
Vacuum Setup (Figure 1)
1. Cut tubing into three pieces of appropriate length.
2. Use one piece of tubing to connect the right side of the vacuum regulator
to the vacuum source.
3. Use another piece of tubing to connect the left side of the vacuum regulator
to the sidearm of the filter flask.
4. Place a rubber stopper with hole into the mouth of the filter flask. Insert a
serological pipette (or comparable) into the hole of the stopper.
5. Snap in the black sealed end of the quick connect fitting into the manifold
base.
6. Finally, use the last piece of tubing to connect the filter flask to the quick
connect fitting of the nozzle of the manifold.
Note: Use of the Aurum vacuum regulator is strongly recommended to
ensure full control of the negative pressure of the manifold.
Fig. 1. Vacuum setup conditions .
Vacuum source Vacuum regulator Filter flask Vacuum manifold
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