Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 23)

Section 9
Troubleshooting Guide
Problem Possible Cause Recommended Solution
Genomic DNA Incomplete DNase I Increase DNase I digest
contamination digest time
Inactive DNase I Store reconstituted
DNase I in a nonfrost-
free freezer. Avoid
freeze-thaw cycles.
Aliquot reconstituted
DNase I for single use
only
Incorrect preparation Use only the DNase
of DNase dilution dilution solution
provided in the kit to
dilute the DNase
Excessive amount of Reduce amount of
starting material starting material used
RNA degradation RNase contamination of DEPC-treat all hand-
user-made solutions made solutions.
and/or plasticware Decontaminate all work
surfaces. See Section
5 for more details
Endogenous RNases Work quickly through
the steps prior to the
addition of lysis solution
Clogging of RNA Excessive amount of Reduce amount of
binding column starting material starting material used
Poor disruption and/or Increase intensity/
homogenization duration of disruption
and/or homogenization
Switch to more intense
disruption and/or
homogenization method
Incomplete digest with Increase duration of
lysozyme or lyticase lysozyme or lyticase
digest. Use fresh
enzyme
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