Bio-Rad Aurum™ Total RNA Mini Kit - Manual (Page 24)

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Problem Possible Cause Recommended Solution
Low eluate volume Insufficient centrifugation Add 1–3 min to the
(<60 µl) time during elution centrifugation time during
elution
Column clogging See problem “Clogging
of RNA binding column”
High eluate volume Low stringency wash Add 1–3 min to the
(>80 µl) carryover in eluate centrifugation time
after the final wash step
Low RNA yield Low amount of Increase starting
starting material material amount up to
the maximum indicated
for the specific starting
material type
Excessive amount of Reduce amount of
starting material starting material used
Poor disruption and/or Increase intensity/
homogenization duration of disruption
and/or homogenization
Switch to more intense
disruption and/or
homogenization method
Incorrect use of wash Add the appropriate
solutions volume of 95–100%
ethanol to the wash
solutions before initial
use
Incorrect preparation of Use only the DNase
DNase dilution dilution solution
provided in the kit
to dilute the DNase
Low sample eluate See problem “Low
volume eluate volumes (<60 µl)”
Inefficient elution Preheat the elution
solution to 70°C in
water bath prior to
the elution step
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