Bio-Rad Nuvia™ IMAC Resin - Manual (Page 8)

4 Bio-Scale Mini Nuvia IMAC Ni-Charged
Nuvia IMAC Cartridges are compatible with the aqueous buffers
most commonly used with IMAC purification techniques.
Table 3. Chemical compatibilities for Nuvia IMAC Ni-Charged
Cartridges.*
Reagent Stability
Chelating agent
EDTA 1 mM
Sulfhydryl reagents
β-mercaptoethanol 20 mM
DTT 10 mM
TCEP 20 mM
Detergents
Nonionic
(Triton, Tween)
2%
Zwitterionic
(CHAPS, CHAPSO)
1%
Denaturing agents
Guanidine-HCl 6 M
Urea 8 M
Other additives
NaCl 2 M (include at least 300 mM NaCl in buffers)
MgCl
2
100 mM (use HEPES to prevent precipitation)
CaCl
2
5 mM (use HEPES to prevent precipitation)
Glycerol 20% (backpressure may increase significantly,
slower flow rates may be required)
Ethanol 20%
* Nuvia IMAC binding capacities are unaffected up to the concentrations given when employing
typical reagents used for histidine-tagged protein purification.