Bio-Rad Nuvia™ IMAC Resin - Manual (Page 7)

Bio-Scale Mini Nuvia IMAC Ni-Charged 3
Table 2. Nuvia IMAC Ni-Charged Resin specifications.
Functional ligand Nitrilotriacetic acid (NTA)
Base bead UNOsphere ™
Particle size range 38–53 μm
Mean particle size 50 μm
Metal ion capacity ≥18 μmol Cu 2+ /ml
Dynamic binding
capacity*
≥40 mg/ml
Recommended flow
rate
480 cm/hr
Maximum operating
pressure
45 psi
Chemical compatibility See Table 3
Storage 4°C to ambient temperature
Storage
recommendations
20% ethanol
Shelf life in 20% ethanol >3 years at ambient temperature
Operational
temperature range
4–40°C
Autoclavability Not autoclavable
* Q10% determination of 1 mg/ml (His)6-tagged pure protein (40 kD). Note: Dynamic binding
capacity will vary from protein to protein.