Bio-Rad Nuvia™ IMAC Resin - Manual (Page 5)

Bio-Scale Mini Nuvia IMAC Ni-Charged 1
Section 1
Introduction
Bio-Scale ™ Mini Nuvia ™ IMAC Ni-Charged Cartridges are
convenient, disposable, prepacked low-pressure chromatographic
columns. Bio-Scale Mini Cartridges offer both increased run-to-
run uniformity and high purity of protein through a patent pending
column design and novel resin technology. Compatible with
aqueous buffers most commonly used for protein purification,
Bio-Scale Mini Cartridges offer improved performance for your
protein separation needs.
Immobilized metal affinity chromatography (IMAC) is an excellent
chromatography technique for purification of histidine-tagged
proteins. The principle of IMAC is based on the affinity histidine
has for metal ions. Side chains on the nitrilotriacetic acid (NTA)
functional ligand selectively bind recombinant histidine-tagged
proteins when the resin is charged with Ni
2+ or other metals. The
advantage of this technique is that proteins can often be purified
close to homogeneity in a single step.
Bio-Scale Mini IMAC Cartridges are packed with Bio-Rad’s
specially designed Nuvia IMAC Ni-Charged Resin. Such
characteristics as the polymeric nature, optimized ligand density,
and open pore structure of the Nuvia IMAC bead result in superb
mechanical strength with high stringency, low nonspecific binding,
and the ability to perform separations at high flow rates.