Bio-Rad Nuvia™ IMAC Resin - Manual (Page 17)

Bio-Scale Mini Nuvia IMAC Ni-Charged 13
Section 10
Regenerating, Cleaning, Sanitizing, and Storing
Protein cross-contamination, frit clogging, and increased
backpressure can result from running a column beyond the
recommended number of uses. After repeated use, a cartridge
may run slower or produce higher backpressure, a result that
should be expected due to the nature of the sample mixture. The
following cleaning and regeneration procedures may be used.
However, we recommend that you dispose of a cartridge after
several uses. To avoid cross-contamination, we recommend that
each cartridge be designated for a single protein. To maintain good
flow properties, we recommend that the cartridges be cleaned
between uses.
For 1 ml cartridges, run the cleaning protocol at 2 ml/min. For 5 ml
cartridges, run the cleaning protocol at 5 ml/min.
High salt/acid cleaning
1. Rinse the cartridge with 2 CV water at 2 ml/min.
2. Wash the cartridge with 5 CV 500 mM NaCl, 50 mM Tris,
pH 8.0 at 2 ml/min.
3. Wash the cartridge with 5 CV 500 mM NaCl, 100 mM NaOAc,
pH 4.5 at 2 ml/min.
4. Rinse the cartridge with 2 CV water at 2 ml/min.
5. Store the cartridge in 20% ethanol at 4°C.
Chaotropic cleaning
1. Rinse the cartridge with 2 CV water at 2 ml/min.
2. Wash the cartridge with 5 CV 6 M guanidine HCl at 2 ml/min.
3. Rinse the cartridge with 2 CV water at 2 ml/min.
4. Store the cartridge in 20% ethanol at 4°C.