Bio-Rad Nuvia™ IMAC Resin - Manual (Page 18)

14 Bio-Scale Mini Nuvia IMAC Ni-Charged
In situations where it is desirable to run different proteins over
the same cartridge, completely sanitize, strip, and recharge the
cartridge between sample runs. Take care when handling and
disposing of metal-containing solutions.
1. Clean the cartridge with 10 CV of 1 M NaOH.
2. Rinse the cartridge with 10 CV water.
3. Strip metal ions with 5 CV of 0.1 M EDTA.
4. Rinse the cartridge with 10 CV water.
5. Recharge the cartridge with 5 CV of 0.1 M nickel sulfate
pH 4.5.
6. Rinse the cartridge with 10 CV water.
7. Store the cartridge in 20% ethanol.