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Bio-Rad Model EP-1 Econo Pump User Manual

Page 16

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7.1 Use of the Econo Pump with the Model 2110
Fraction Collector

The Econo Pump is capable of controlling the Model 2110 Fraction

Collector. The pump must be calibrated in order to run a fraction collector
program. See Section 6 for calibration instructions.

Use System Cable 1 (catalog number 731-8261) to connect the Econo

Pump to the fraction collector. Connect the 9-pin “D” connector to rear of
the Model 2110, and the mini-DIN plug to the rear port of the Econo Pump
with the collector icon. (See Figure 4.2.)

To program a simple time-based collection method, follow these

instructions.

1. Press the Fraction Collector key on the pump front panel

to enter the fraction collector Edit mode. Note that the
fraction size and ml indicator lights are flashing, prompt-
ing entry of fraction size. Enter the fraction size in 0.1 ml
increments using the Arrow keys. After fraction size is
selected, press the Fraction Collector key once more to
confirm the fraction size.

2. Enter a void volume (Vo) in 1 ml increments using the

Arrow keys. This feature allows the user to begin col-
lecting fractions only after a pre-set volume of liquid
(i.e., the void volume) has passed through the column.
Press the Fraction Collector key to confirm this value. If
a void volume is not desired, enter 0.

Note: The optional Model SV-3 Diverter Valve (catalog
number 731-8235) is required to use the void volume
feature of the Model EP-1 Econo Pump.

3. Select the total run volume (Vt ) in 1 ml increments

using the Arrow keys.* If zero is entered and confirmed,
the fraction collection scheme is disabled. To resume
data entry, see Step 1 above. All values previously
entered remain in the memory until other values are
entered to replace them.

*

Note: The Econo Pump will automatically shut off upon
reaching the total run volume (Vt ).

ml

min

ml/min

%

V

V

t

o

ml

min

ml/min

%

V

V

t

o

ml

min

ml/min

%

V

V

t

o

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