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Bio-Rad Model EP-1 Econo Pump User Manual

Page 14

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Note: Overtightening the platen adjustment screw will reduce flow rate
and shorten tubing life. If the platen is too loose, flow rate will decrease
as backpressure increases.

Section 6
Stand Alone Operation

The Model EP-1 Econo Pump functions as a stand-alone pump. By

specifying the pump tubing size, it allows you to enter a flow rate rather
than a nominal pump speed setting. (An uncalibrated Econo Pump will
display pump speed as percent of maximum rpm.) There are two ways to
calibrate the pump: through a preset tubing size or through a user
calibration procedure.

Flow Rate Calibration with Pre-Selected Tubing Sizes (0.8, 1.6,
and 3.2 mm ID)

1. With the pump in Stop mode, install tubing as described

in Section 5.2.

2. Press the key in the lower left-front of the pump panel

corresponding to the pump calibration feature, and
continue pressing until the indicator light corresponding
to either 0.8, 1.6, or 3.2 mm ID tubing is lit.

3. The LED display reads ml/min for any of these pre-pro-

grammed tubing settings. If improved flow rate accuracy
is desired, see User Calibration Procedure below.

4. The pump is now automatically calibrated for the specific

tubing size. Press the Run/Stop key to start flow. Increase
or decrease the pump speed with the Arrow keys.

User Calibration Procedure

1. Install tubing as described in Section 5.2. Connect

pump inlet to a container of water or buffer. Connect a
length of tubing to the pump outlet.

2. Run pump to purge air from tubing, then stop pump.

Place the end of the outlet tubing in an empty graduated
cylinder.

ml

min

ml/min

%

ml

min

ml/min

%

0.8

1.6

3.2

CAL

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