Bio-Rad Model EP-1 Econo Pump - Manual (Page 15)

3. Press Calibration key until “CAL” is displayed. Press
the Run/Stop key. Pump will run for 5 minutes at 25%
of maximum speed, and the display will count down
time.
4. When pump stops, read the volume of fluid on the
graduated cylinder. Using the Arrow keys, enter this
amount on the display. Alternatively, when a suitable
volume of liquid has been collected, pressing Run/Stop
will turn off the timer and stop the pump. Enter volume
collected as described.
5. When the value on the display matches the volume
collected in the graduated cylinder, press Calibration.
The “CAL” indicator light will glow steadily, indicating
that the pump is calibrated. DO NOT select a tubing
size at this point, even if you are using one of the standard
sizes. The pump will “remember” the calibration key
even if it is switched off or disconnected from the
power line.
6. Set desired flow rate using arrow keys.
Note: Flow rate depends on condition of tubing, platen
adjustment, and back pressure. For maximum flow rate
accuracy it may be necessary to recalibrate as the tubing
ages, when the tubing is replaced, if the platen is read-
justed, or if back pressure changes significantly.
Section 7
Operation with other Instruments
The Model EP-1 Econo Pump can be used with other chromatography
components (Model 2110 and Model 2128 fraction collectors), or with the
BioLogic System, the high resolution liquid chromatography system. Each
of these applications is discussed below.
0.8
1.6
3.2
CAL
0.8
1.6
3.2
CAL
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