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1 dynaloop sample loop description, 2 operation – Bio-Rad BioLogic Sample Loading Loops User Manual

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Section 1
Introduction

1.1 DynaLoop Sample Loop Description

The DynaLoop sample loop is a large-volume sample injection loop for use with the

BioLogic DuoFlow

system. Available in 25 ml and 90 ml sample capacities, the DynaLoop

is used with the automated AVR7-3 injection valve.

1.2 Operation

The DynaLoop consists of five major components (Figure 1):

Sample end assembly

Buffer end assembly

Glass tube graduated in 1 ml increments

Sliding seal assembly

Protective plastic jacket

Fig. 1. DynaLoop 25 and DynaLoop 90 sample loops.

The sliding seal assembly divides the glass tube into two distinct chambers:

Sample chamber
This end connects to port 3 on the AVR7-3 injection valve.

Buffer chamber
This end connects to port 6 on the AVR7-3 injection valve.

The DynaLoop sample loop delivers sample to the column in a four-phase process:

Load phase

Injection phase

Flush phase

Reload phase

Sample end
assembly

Sliding seal
assembly

Glass tube

Buffer end
assembly

Plastic jacket

0

5

10

15

20

25

0

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

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