Bio-Rad BioLogic Sample Loading Loops - Manual (Page 16)

4.2 Cleaning and Storage
Refer to Figure 9 as you follow these instructions.
Fig. 9. DynaLoop cleaning.
Cleaning and Storage
With the DynaLoop in the fluid path, use the BioLogic DuoFlow pump to deliver the
cleaning solutions to the DynaLoop.
1. Rinse with 1 volume of a dilute acid such as 0.1 M acetic acid.
2. Flush with 10 volumes of HPLC-grade water.
3. Rinse with 1 volume of 0.1 M sodium hydroxide. Flush immediately with 10 volumes of
HPLC-grade. (Prolonged contact with sodium hydroxide will etch the glass tube.)
4. Rinse with a solution of 10% methanol and 90% HPLC-grade water and dry thoroughly
before use.
For storage periods longer than a few days, store the unit disassembled at room
temperature.
Disassembly
1. Turn off the BioLogic DuoFlow pump. Remove the DynaLoop from the fluid path.
2. Disassemble the unit by unscrewing the end caps and removing the glass tube.
3. Using a blunt plastic instrument, push the sliding seal assembly out of the glass tube.
4. To disassemble the sliding seal assembly, hold the body of the assembly in one hand and
twist the cap that retains the O-ring counterclockwise. The check valve components
(spring, guide, round rubber ball), and O-ring can now be removed and inspected.
Sliding seal
assembly
cap, sample
O-ring
Shield, 25 ml
Shield, 90 ml
Glass tube
assembly, 25 ml
Glass tube
assembly, 90 ml
End cap, buffer
O-ring
Filter
0 5 10 15 20 25
Housing
O-ring, coated
Ball, rubber
Guide, check valve
Spring, PTFE coated
Cap
12