Bio-Rad BioLogic Sample Loading Loops User Manual

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Load Sample Manually (via a syringe)

1. With the injection valve in the INJECT position, allow the sliding seal to contact the

sample end fitting. Set the valve to the LOAD position. Load sample into the DynaLoop
by using a syringe at the filler port (port 2). Hint: use a 1/4-28 – Female Luer adaptor at
port 2 (part no. 910-4159). As sample is loaded into the loop it displaces the buffer that
was used to purge the unit.

2. When sufficient volume of sample for a run or series of runs is loaded into the

DynaLoop, stop the filling process. For a series of partial volume injections after one
loading sequence, fill the DynaLoop with about 2 percent extra sample.

Leave the syringe inserted in the injection port to minimize the introduction of air into
the DynaLoop.

3. The sample is now ready for injection.

Load Sample Automatically

1. To load the sample automatically using an auxiliary pump such as the Model EP-1 or

Econo gradient pump, you must select AUX Pump in the BioLogic setup editor.

2. In the Protocol screen, program an Isocratic Flow step that is long enough for the

auxiliary pump to load the desired quantity of sample into the DynaLoop. For example,
if you are loading 25 ml of sample at an auxiliary pump flow rate of 5 ml/min, you will
need at least a 5=minute step prior to the sample loading step. Otherwise the protocol
will fail the automatic validation done prior to the run.

The flow rate of the BioLogic DuoFlow pump is not critical, so you may set a low flow
rate (e.g., 0.1 ml/min) to minimize buffer waste. It is important that either the time or
volume length of this first step is of sufficient duration to allow the auxiliary pump to fill
the DynaLoop.

3. In the Protocol screen, select Load/Inject Sample to program the BioLogic DuoFlow

system to automatically fill and inject the DynaLoop sample. From the Load/Inject
Sample window,

Select the Dynamic Loop as the type of loop to be used.

Select Fill Before Inject. This instructs the auxiliary pump to load the sample into
the DynaLoop.

Note when using the EP-1:

The auxiliary pump flow rate is not under the control of the BioLogic DuoFlow. The
flow rate is used by the system when validating the protocol before the run.
Because the BioLogic DuoFlow system only starts/stops the EP-1, the correct flow
rate must be set at the EP-1. It is recorded in the yellow data entry boxes of the Fill
Sample section of the Load/Inject Sample dialog box. Note that the rinse function
is not available when the DynaLoop is being used.

Note when using the Econo gradient pump:

The auxiliary pump flow rate is under the control of the BioLogic DuoFlow. Note
that the rinse function is not available when the DynaLoop is being used.

In the Fill Sample Loop area of the window, select the sample to be loaded and
enter its volume and the flow rate of the auxiliary pump.