Bio-Rad BioLogic Sample Loading Loops - Manual (Page 15)

d. In the Inject Sample area of the window, select the Injection Buffers, the buffer
composition, the flow rate of the BioLogic DuoFlow workstation pumps, and the
sample volume to be injected onto the column.
The BioLogic DuoFlow system will now automatically control the loading and injection
of the sample. Note that the rinse function is not available when the DynaLoop is being
used.
4. Continue writing your desired separation protocol.
The AVR7-3 Injection Valve Function
During the run, the AVR7-3 injection valve functions as follows (Figure 6):
• Load. While in this position the valve connects ports 5 and 4 for equilibration of the
column and for sample elution. In this position, sample loop is loaded to the desired
volume via port 2 and buffer is expelled from the dynamic loop through port 1.
• Inject. While in this position the valve connects ports 5 and 6 and ports 3 and 4 for
applying the sample onto the column. The flow from the workstation pump forces the
sliding piston to expel the sample onto the column.
• Purge. While in this position the valve connects ports 5 and 7 and allows purging or
buffer changes of the workstation pump without the need to remove the column from
the system.
Section 4
Care and Maintenance
4.1 Troubleshooting
Table 1. Troubleshooting the DynaLoop
Problem Possible Cause Corrective Action
High backpressure Plugged filter Replace filter
when loading or injecting (Section 4.3)
Leakage at the compression Loose or worn ferrules Tighten or replace ferrules
screw end fittings
Leakage into the plastic Worn O-rings on Replace O-rings
shield sample or buffer end (Section 4.4)
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