Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits User Manual
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Section 1
Introduction
Acute phase proteins are a class of proteins that have altered levels in
response to inflammation and are thus relevant biomarkers in many
disease processes. Typically, initial release of cytokines at the site of
damage, most notably IL-1, IL-6, IL-8 and TNF-Ͱ, triggers the liver to
release a number of acute phase proteins into the bloodstream. In turn,
the increased levels of these proteins stimulate the release of more
cytokines, presumably to initiate processes to prevent further tissue
damage. Positive acute phase proteins—those that increase in response
to tissue damage—are associated with infection, physical trauma, cardiac
injury, diabetes, cancer, and other diseases.
Bio-Plex Pro™ assays are bead-based multiplex assays that allow the
measurement of nine positive acute phase biomarkers in diverse matrices,
including serum, plasma, and tissue culture supernatant. The biomarkers
selected are circulating proteins that are known to be present in elevated
levels during the acute phase response. The multiplexing feature makes it
possible to quantify the levels of multiple target proteins simultaneously in
a single well of a 96-well microplate in just 3 hours with as little as 5 µl of
sample.
As one of the most recent additions to the Bio-Plex family of products,
Bio-Plex Pro assays incorporate magnetic beads into their design. The
magnetic beads allow the use of an assay protocol similar to nonmagnetic
Bio-Plex assays, with the option of using magnetic separation during wash
steps instead of vacuum filtration. The magnetic washing option also
enables automated assay preparation.
Bio-Plex Manager™ software is recommended for Bio-Plex Pro acute phase
assays. Instructions for Luminex xPONENT software are also provided. For
instructions using other xMAP system software packages, contact Bio-Rad
Technical Support or your Bio-Rad Field Applications Scientist.
For a current listing of Bio-Plex Pro acute phase assays, visit us on the
Web at
www.bio-rad.com/bio-plex/
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