Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 23)

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Section 10
Data Acquisition
Bio-Plex Manager™ software is recommended for Bio-Plex Pro™ acute
phase assays. Refer to the details in the appropriate section below for
your xMAP system software package. Instructions for Luminex xPONENT
software are also provided.
For instructions using other xMAP system software packages, contact
Bio-Rad Technical Support or your Bio-Rad Field Applications Scientist.
NOTE: Bio-Plex Pro acute phase assays are analyzed using the low
RP1 target value (low PMT). Be sure to follow the instructions for your
software version on how to calibrate and analyze data.
NOTE: To minimize protocol setup, lot-specific Bio-Plex Pro acute
phase assay protocols for Bio-Plex Manager version 4.0 and higher are
available for download at
www.biorad.com/bio-plex/standards
Bio-Plex Manager Software Version 5.0
Prepare System
1. Empty the waste bottle and make sure that there is sufficient sheath
fluid in the bottle or cube before proceeding.
2. Turn on the reader, XY platform and HTF (if included). Allow the
system to warm up for 30 min.
3. Select Start up and follow the instructions to prepare the reader
to acquire data. If the system is idle for 4 hr without aquiring data, the
lasers will automatically turn off. To reset the 4-hour countdown, select
Warm up
and wait for the optics to reach operational temperature.
Calibrate With Low RP1 Target Value
Calibrate using Bio-Plex calibration beads and low RP1 target values on the
CAL2 bottle. This software version requires calibration only with the low