Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 31)

Acquire Data
1. Shake the assay plate at 900 rpm for 30 sec immediately before
acquiring data. Failure to do so will result in increased data
acquisition time due to bead settling.
2. Visually inspect the plate and ensure that the assay wells are filled with
buffer prior to placing the plate in the Bio-Plex microplate platform.
3. Slowly remove the sealing tape and any plate cover before placing
the plate in the reader.
4. Select Step 7 (Run Protocol):
a) Choose 100 beads per region.
b) In Advanced Settings, set the bead map to 25 region.
c) In Advanced Settings, set the sample size to 50 µl.
d) In Advanced Settings, confirm that the DD gate are set to 5,000
(low) and 32,000 (high).
NOTE: These settings are already selected in preset
downloaded protocols.
e) Select Start and save the protocol (.pbx) file. Then follow the
instructions for data acquisition.
5. If acquiring data from ore than one plate, empty the waste bottle and
refill the sheath bottle after each plate (if HTF not present). Select Wash
Between Plates and follow instructions for fluidics maintenance.
Then repeat the Prepare Protocol and Acquire Data steps.
NOTE: Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
6. When data acquisition is complete, select Shut Down and follow
the instructions.
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