Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 17)

Section 8
Control Preparation
Two vials of lyophilized acute phase controls are provided in each
multiplex Bio-Plex Pro™ acute phase assay kit. This section provides
instructions on how to reconstitute the Iyophilized control. The product
insert provided with the assay lists the concentration ranges of the
prepared controls.
Prepare Acute Phase Controls
To ensure optimal assay performance, the acute phase controls should be
prepared in a manner consistent with that used to prepare the acute phase
standards.
1. Gently tap the glass vial containing the lyophilized acute phase
control on a solid surface to ensure the pellet is at the bottom.
2. Refer to the steps below for reconstituting the 5-plex and 4-plex
controls. Use the standard/control diluent prepared in the previous
section (Section 7, step 2). Do not use assay buffer to reconstitute
the controls.
5-plex control:
Reconstitute one vial of lyophilized control with
1 ml of 5-plex standard/control diluent. This solution will be used
directly in the assay; no additional dilutions are required.
4-plex control:
Reconstitute one vial of lyophilized control with
2 ml of 4-plex standard/control diluent. This solution will be used
directly in the assay; no additional dilutions are required.
3. Gently vortex 1-3 sec and incubate on ice for 60 min. For optimal
assay performance, be consistent with the incubation time.
4. Expected concentration ranges of the prepared controls are provided
in the standard and control values product insert.
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