Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits User Manual
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Possible Causes
Possible Solutions
High Background Signal
Incorrect buffer was used
(for example, assay buffer
used to dilute standards)
Use sample matrix or serum-based
diluent to dilute standards.
Spiked 0 pg/ml wells by mistake
Be careful when spiking standards.
Do not add any antigens in the
0 pg/ml (blank) point.
Streptavidin-PE incubated
too long
Follow the procedure incubation
time.
Poor Recovery
Expired Bio-Plex reagents were
used
Check that reagents have not
expired. Use new or unexpired
components.
Incorrect amounts of components
were added
Check your calculations and be
careful to add the correct volumes.
Microplate shaker set to an
incorrect speed
Check the microplate shaker speed
and use the recommended setting.
Setting the speed too high may
cause splashing and contamination.
Use the recommended plate shaker.
Pipetting technique
Pipet carefully and slowly when
adding standards, samples,
detection antibodies, and
streptavidin-PE, especially when
using a multichannel pipet. Use a
calibrated pipet. Change pipet tip
after every volume transfer.