Bio-Rad Bio-Plex Pro Human Acute Phase Reagent and Diluent Kits - Manual (Page 18)

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Section 9
Assay Instructions
The following instructions apply to both the 5-plex and 4-plex assays. All
of the necessary components are provided premixed for ease of use.
Plan Experiment
1. Assign which wells of a 96-well plate will be used for each standard,
control, and sample (see the example plate layout below).
2. Determine the total number of wells that will be used in the assay.
Include a 25% excess (or add 2 wells for every 8 wells used) to
ensure that enough diluted coupled beads, detection antibodies,
and streptavidin-PE are prepared.
Example Plate