Bio-Rad Rat Diabetes Assays - Manual (Page 8)

6
Prewet wells
(for filter plate only)
Add 50 μl 1x beads to wells
Wash 2 x 100 μl
Add 50 μl standards, blank, samples
incubate 1 hr at RT with shaking at 850 rpm
Wash 3 x 100 μl
Add 25 μl 1x detection antibody, incubate
30 min at RT with shaking at 850 rpm
Wash 3 x 100 μl
Add 50 μl 1x streptavidin-PE, incubate
10 min at RT with shaking at 850 rpm
Wash 3 x 100 μl
Resuspend in 125 μl assay buffer,
shake at 850 rpm for 30 sec
Read plate on Bio-Plex system
Assay Workflow