Bio-Rad Rat Diabetes Assays - Manual (Page 3)

Introduction
Bio-Plex Pro ™ diabetes assays are magnetic bead–based multiplex assays
designed to measure multiple diabetes-related biomarkers in a minimal
volume of matrix such as serum, plasma, tissue culture supernatant,
or other biological fluids. The biomarkers chosen for these assays are
circulating proteins involved in the regulation of glucose metabolism.
These multiplex assays are configured for the detection of various
metabolic markers in human, mouse, and rat samples. The multiplex
assays for human matrices were tested and found to be cross-reactive to
at least four common non-human primate (NHP) species, including rhesus,
cynomolgus, baboon, and marmoset. The degree of cross-reactivity was
profiled according to the ability of each assay to detect these metabolic
markers in the sera and mitogen-stimulated peripheral blood mononuclear
cell (PBMC) culture supernatant of these animals.
The high level of gene homology across the major NHP species may
allow the NHP diabetes assays to be used in other species, such as
chimpanzees, African green monkey, and pigtail macaques. However,
assay performance has not been specifically evaluated in these animals.
Bio-Plex Pro assays enable researchers to quantify multiple protein
biomarkers in a single well of a 96-well plate in 3–4 hours. These robust
immunoassays require as little as 12.5 μl serum or plasma or 50 μl cell
culture supernatant or other biological fluid. The use of magnetic (MagPlex)
beads allows researchers to automate wash steps on a Bio-Plex Pro (or
similar) wash station. Magnetic separation offers greater convenience,
productivity, and reproducibility compared to vacuum filtration.
For more information please visit www.bio-rad.com/bio-plex.
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Cross-Reactivity with Non-Human Primates
African green Pigtail
Rhesus Cynomolgus Baboon Marmoset Chimpanzee monkey macaque
Tested • • • •
Not tested • • •