Controls, And samples – Bio-Rad Rat Diabetes Assays User Manual
Page 32
30
Table 21. Bead regions for compatible Bio-Plex Pro cytokine assays. Assays shown have
bead regions that are non-overlapping with diabetes assays.
3. Click Format Plate and format the plate according to the plate layout
created in Section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 6).
a. Select
the
Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
blanks
B
, controls
C
, and samples
X
.
Human Cytokines
Mouse Cytokines
Rat Cytokines
Group I Bead Group II Bead Group I Bead Group II Bead Group I Bead
Region
Region
Region Region
Region
IL-1b
39
IL-1a
63
IL-1a
53 IL-15
42
IL-1a 21
IL-1ra
25
IL-2Ra 13
IL-1b
19 IL-18
20
IL-1b 28
IL-2
38
IL-3
64
IL-2
36 Basic FGF 25
IL-2 22
IL-4
52
IL-12p40 28
IL-3
18 LIF
45
IL-4 33
IL-5
33
IL-18
42
IL-4
39 M-CSF
26
IL-5 52
IL-6
19
HGF
62
IL-5
52 MIG
44
IL-6 56
IL-7
74
IFN-a2
20
IL-6
38 MIP-2
27
IL-7 38
IL-8
54
LIF
29
IL-9
33 PDGF-BB 35
IL-10 19
IL-9
77
M-CSF
67
IL-10
56 VEGF
47
IL-12p40 76
IL-10
56
MIF
35
IL-12p40
76
IL-12p70
78
IL-12p70
75
b-NGF
46
IL-12p70
78
IL-13
15
IL-13
51
TNF-b
30
IL-13
37
IL-17
72
IL-15
73
TRAIL
66
IL-17
72
IL-18
20
IL-17
76
Eotaxin
74
EPO
14
Eotaxin
43
G-CSF
54
G-CSF
54
Basic FGF 44
GM-CSF
73
GM-CSF 37
G-CSF
57
IFN-g
34
GRO/KC
57
GM-CSF
34
KC
57
IFN-g
34
IFN-g
21
MCP-1
(MCAF)
51
M-CSF
26
IP-10
48
MIP-1a
77
MIP-1a
77
MCP-1
53
MIP-1b
75
MIP-2
27
MIP-1a
55
RANTES
55
MIP-3a
36
MIP-1b
18
TNF-a
21
RANTES
55
PDGF-BB 47
TNF-a
43
RANTES
37
VEGF
47
TNF-a
36
MCP-1 51
VEGF
45
* Mouse group III cytokines have not been tested for cross reactivity with diabetes or other panels.
Group III*
Bead
Region
IL-17F 28
IL-21 14
IL-22 15
IL-23p19 61
IL-25 67
IL-27p28 43
IL-31 29
IL-33 13
MIP-3a 12