Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 31)

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Table 13. Troubleshooting guide (continued).
Problem Possible Causes Possible Solutions
Low bead count Cell debris in lysate Centrifuge at 15,000 x g for 10 min
not cleared at 4°C to remove cellular debris
Assay plate not shaken Shake plate at 900–1,100 rpm
enough prior to reading for 30 sec before data acquisition
Clogged reader Refer to the troubleshooting
guide in the Bio-Plex system
hardware instruction manual
(bulletin 10005042)
Miscalculation of bead dilution Check the calculations and be
careful to add the correct volumes
Clumping of stock beads Vortex the stock vial at different
in vials angles for 30 sec at medium speed
before aliquoting beads
Vacuum setting too high Adjust pressure to –1 to –3" Hg.
during suction of assay plate Generally, 100 μl liquid should
take 3–4 sec to clear from the well
Reader needle height Adjust the needle height to coincide
incorrect with the plate type provided in the kit
Beads lost Use recommended magnetic
washer settings with correct
soaking time
continues