Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays User Manual

Page 27

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10. Click Enter Sample Info and enter sample information and the

appropriate dilution factor if any.

11. Click Run Protocol and confirm that the assay settings are correct.

Refer to Table 11 for the recommended RP1 (PMT) setting.
Protocols using alternative settings should be validated by the
end user.

b. Confirm that data acquisition is set to 50 beads per region.

In Advanced Settings, confirm that the bead map is set to
100 region, the sample size is set to 50 µl, and the doublet
discriminator (DD) gates are set to 5,000 (Low) and 25,000 (High).
In Bio-Plex Manager software versions 4.0, 4.1, 4.1.1, and 5.0,
check Override Gates and set the DD gate values as indicated.

Select

Start, name and save the .rbx file, and begin data

acquisition. The Run Protocol pop-up screen will appear. Click
Eject/Retract to eject the plate carrier.

Fig. 3. Plate formatting.