Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 23)

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Instrument RP1 (PMT) DD Gates Bead Events
Bio-Plex 100, 200* High 5,000 (low), 25,000 (high) 50
Bio-Plex 3D* Enhanced Select MagPlex beads 50
Bio-Plex MAGPIX* N/A, use default instrument settings
* Or similar Luminex-based system.
36. To resuspend beads for plate reading, add 125 µl resuspension
buffer to each well. Cover the plate with a new sheet of sealing tape
and shake at 900–1,100 rpm for 30 sec.
36. Slowly remove the sealing tape and place the plate on the reader to
acquire data.
Table 11. Read the plate using the appropriate instrument settings.
5. Read the Plate
Bio-Plex Manager software is recommended for all Bio-Plex Pro assay
data acquisition and analysis. Instructions for Luminex xPONENT software
are also included. For instructions on using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Instrument
Start up and calibrate the Bio-Plex 100/200 or similar system with Bio-Plex
Manager software prior to setting up the assay. The calibration kit should
be run daily or before each use of the instrument to standardize the
fluorescent signal. To prepare either a Bio-Plex 3D or Bio-Plex
® MAGPIX ™
reader, consult its respective user manual. For instructions on using other
xMAP system software packages, contact Bio-Rad Technical Support.
The validation kit should be run monthly to ensure optimal performance
of fluidics and optics systems. Refer to either the software manual or
online Help for instructions on how to conduct validation.